Making NMR sample

Spectrometer ---- NMR Tube ---Total sample volume --- Volume buffer --- Volume Chenomx 5 mM DSS-d6/D2O/NaN3 solution

 nmr500             535PP                500 uL             475 uL               25 uL      this leads to a 0.25 mM DSS sample with 5% D2O
 nmr600             535PP                500 uL             475 uL               25 uL
 ibd600             535PP                600 uL             570 uL               30 uL
 nanuc500           535PP                500 uL
 nanuc800           535PP                700 uL
 mrdc600      special 4" NMR tube  (see Deryck Webb at NANUC)